Document 0567
 DOCN  M9480567
 TI    Flow cytometric analysis of natural killer cell function as a clinical
       assay.
 DT    9410
 AU    Hatam L; Schuval S; Bonagura VR; Department of Pediatrics, Schneider
       Children's Hospital, Long; Island Jewish Medical Center, New Hyde Park,
       NY 11042.
 SO    Cytometry. 1994 May 1;16(1):59-68. Unique Identifier : AIDSLINE
       MED/94307086
 AB    The 51Cr release assay has been the method of choice in analyzing
       natural killer cell (NK) function. Previous FCM cytotoxicity assays of
       NK activity have had numerous disadvantages that discouraged clinicians
       from attempting to evaluate NK function by flow cytometry. We
       demonstrate the effectiveness of using PKH-26, a stable membrane dye, to
       label the K562 target cells and propidium iodide intercalation into
       killed target cell DNA to determine the percentage of target cells
       killed by effector NK cells from the peripheral blood or bone marrow.
       This method compares favorably with the 51Cr release assay and is
       quicker and easier to perform. The percentage of cytotoxicity of NK
       cells (CD3- CD56+ and/or CD16+) from 10 normal subjects and 10
       HIV-infected children are reported to demonstrate the feasibility of
       studying NK function in clinical populations by FCM. The potentiation of
       cytolysis by alpha-interferon and interleukin 2 in vitro was also
       compared between these two study groups. In addition, a patient whose
       leukemic blasts expressed CD56+ was also studied for NK activity using
       this flow cytometric assay. The benefits of using this flow cytometric
       approach to clinically assess NK function are discussed.
 DE    Adolescence  Adult  Child  Child, Preschool  Chromium
       Radioisotopes/DIAGNOSTIC USE  Cytotoxicity Tests, Immunologic  *Flow
       Cytometry  Fluorescent Dyes/DIAGNOSTIC USE  Human  HIV
       Infections/IMMUNOLOGY  Immunophenotyping  Killer Cells,
       Natural/IMMUNOLOGY/*PHYSIOLOGY  Leukemia, Myelocytic, Acute/IMMUNOLOGY
       Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).